백서 두개골 결손에서 rhBMP-2와 다양한 carrier의 골재생 유도효과
Effects of rhBMP-2 with various carriers on bone regeneration in rat calvarial defect
이서경, 엄태관, 김지선, 최성호, 조규성, 김창성, 채중규, 김종관, 강은정,
소속 상세정보
이서경 ( Lee Seo-Kyoung ) - 국민건강보험공단 일산병원 치주과
엄태관 ( Eom Tae-Gwan ) - 오스템 임플랜트 연구소
김지선 ( Kim Ji-Sun ) - 국민건강보험공단 일산병원 치주과
최성호 ( Choi Seong-Ho ) - 연세대학교 치과대학 치주과학교실
조규성 ( Cho Kyoo-Sung ) - 연세대학교 치과대학 치주과학교실
김창성 ( Kim Chang-Sung ) - 연세대학교 치과대학 치주과학교실
채중규 ( Chae Jung-Kiu ) - 연세대학교 치과대학 치주과학교실
김종관 ( Kim Chong-Kwan ) - 연세대학교 치과대학 치주과학교실
강은정 ( Kang Eun-Jung ) - 오스템 임플랜트 연구소
KMID : 0363020080380020125
Abstract
Purpose: Bone morphogenetic protein (BMP) is a potent differentiating agent for cells of the osteoblastic lineage. It has been used in the oral cavity under a variety of indications and with different carriers. However, the optimal carrier for each indication is not known. This study evaluated the bone regenerative effect of rhBMP-2 delivered with different carrier systems.
Materials and Methods: 8 mm critical-sized rat calvarial defects were used in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each. Two groups were controls that had no treatment and absorbable collagen membrane only. 4 groups were experimentals that contained rhBMP-2 only and applied with absorbable collagen sponge(), , Bio- each. The histological and histometric parameters were used to evaluate the defects after 2- or 8-week healing period. The shape and total augmented area were stable in all groups over the healing time.
Results: New bone formation was significantly greater in the rhBMP-2 with carrier group than control group. rhBMP-2/ACS was the highest in bone density but gained less new bone area than rhBMP-2/ and rhBMP-2/Bio-. The bone density after 8 weeks was greater than that after 2 weeks in all groups. However, rhBMP-2 alone failed to show the statistically significant difference in new bone area and bone density compared to control group. Also and Bio- particles remained after 8 weeks healing period.
Conclusion: These results suggest that rhBMP-2 with carrier system is an excellent inductive agent for bone formation and we can use it as the predictable bone tissue engieering technique. Future study will likely focus on the kinetics of BMP release and development of carriers that is ideal for it.
키워드
Bone regeneration;carrier;recombinant human bone morphogenetic protein-2;rat calvarial defect
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